Equipment
Cell sorters
Beckman Coulter Moflo Astrios
System: Jet-in-air
Sorting proficiency: 6-way, combination of 5-50ml tubes, 96-well plate and 384-well plate
Enhanced forward scatter (eFSC) for improved overall particle size range with resolution up to 0.2um
5 lasers, 20 colors
|
Excitation laser |
Emission wavelength |
Common dyes/antibodies |
|
355nm (UltraViolet) |
448/59 |
Alexa Fluor 350 |
|
526/52 |
BUV496, Qdot 525, Qdot 545 |
|
|
620/29 |
BUV615 |
|
|
692/75 |
BUV661 |
|
|
405nm (Violet) |
405/10 |
SSC |
|
448/59 |
CFP, DAPI, Pacific Blue, BV421, V450 |
|
|
526/52 |
Qdot525 |
|
|
620/29 |
Qdot625 |
|
|
671/30 |
Qdot655 |
|
|
710/45 |
Qdot705 |
|
|
795/70 |
Qdot800 |
|
|
488nm (Blue)
|
488/6 |
SSC |
|
513/26 |
FITC, GFP, AF488 |
|
|
710/45 |
PerCP, PerCP-Cy5.5 |
|
|
561nm (Yellow Green) |
579/16 |
PE, DsRed |
|
614/20 |
PE-Texas Red, PE-CF594, PI, mCherry |
|
|
664/22 |
PE-Cy5, 7AAD |
|
|
692/75 |
PE-Cy5.5, mPlum |
|
|
795/70 |
PE-Cy7 |
|
|
640nm (Red) |
671/30 |
APC, AF647 |
|
722/44 |
AF700 |
|
|
795/70 |
APC-Cy7, APC-H7 |
Analysers
BD LSR Fortessa
4 lasers, 16 colors
|
Excitation laser |
Emission wavelength |
Common dyes/antibodies |
|
|
Configuration 1 |
Configuration 2 |
||
|
405nm (Violet) |
450/50 |
450/50 |
Pacific Blue, BV421, |
|
525/50 |
525/50 |
CAS Y, AmCyan, V500 |
|
|
540/30 |
Qdot 565 |
||
|
585/15 |
Qdot 585 |
||
|
610/20 |
610/20 |
BV605, BV570, Qdot 605 |
|
|
670/30 |
670/30 |
BV650, Qdot 655 |
|
|
710/50 |
BV711, Qdot705 |
||
|
780/60 |
BV786, Qdot800 |
||
|
488nm (Blue)
|
488/10 |
SSC |
|
|
530/30 |
FITC, GFP, AF488 |
||
|
710/50 |
PerCP, PerCP-Cy5.5 |
||
|
561nm (Yellow Green) |
582/15 |
PE |
|
|
610/20 |
PE-Texas Red, PI, mCherry |
||
|
670/30 |
PE-Cy5, 7AAD |
||
|
710/50 |
PE-Cy5.5 |
||
|
780/60 |
PE-Cy7 |
||
|
640nm (Red) |
670/14 |
APC, AF647 |
|
|
730/45 |
AF700 |
||
|
780/60 |
APC-Cy7, APC-H7 |
||
Beckman Coulter CytoFLEX LX B-R-V-Y-U-I
6 lasers, 21 colors
|
Excitation laser |
Emission wavelength |
Common dyes/antibodies |
|
355 (UV) |
405/30 |
BUV395 |
|
525/40 |
BUV496, Qdot 525, Qdot 545 |
|
|
675/30 |
BUV661, Hoechst Red |
|
|
405nm (Violet) |
450/45 |
Pacific Blue, BV421, Zombie Violet |
|
525/40 |
AmCyan, V500, BV510, Zombie Aqua, LIVE/DEAD Fixable Aqua |
|
|
610/20 |
BV605, eFlour 625NC, Qdot 605 |
|
|
660/10 |
BV650, Qdot 655 |
|
|
763/43 |
BV785, BV786, Qdot 800 |
|
|
488nm (Blue)
|
525/40 |
FITC, GFP, AF488, CFSE, Zombie Green |
|
610/20 |
PI, Mitosox |
|
|
690/50 |
PE-Cy5, PE-Cy5.5, PerCP, PerCP-Cy5.5 |
|
|
561nm (Yellow Green) |
585/42 |
PE, AF546, Cy3, DsRed, dTomato, |
|
610/20 |
PE-Texas Red, PE-CF594, PI, mCherry |
|
|
675/30 |
PE-Cy5, mPlum |
|
|
710/50 |
PE-Cy5.5, PE-Cy5, PE-AF680, PerCP, PerCP-Cy5.5 |
|
|
763/43 |
PE-Cy7 |
|
|
638nm (Red) |
660/10 |
AF633, AF635, AF647, APC, Cy5, eFluor 660, |
|
712/25 |
AF680, AF680-APC, AF680-R-PE, AF700-APC, AF700 |
|
|
763/43 |
AF750-APC, APC-eFlour 780, APC-Fire 750, APC-Cy7 |
|
|
808nm (Infrared) |
840/20 |
AF750, AF790, CF790 |
|
885/40 |
PF840 |
NanoFCM
Detects only nanoparticles below 1μm
|
Excitation laser |
Emission wavelength |
Common dyes/antibodies |
|
488nm (Blue) |
488/10 |
SSC |
|
525/40 |
FITC, GFP |
|
|
580/40 |
PE |
|
|
613/18 |
PE-Texas Red, PI, mCherry |
|
|
640nm (Red)
|
670/30 |
APC, AF647 |
|
710/40 |
AF700 |
|
|
783/56 |
APC-Cy7 |
Magnetic Cell Sorter
Miltenyi SuperMACS separator
Magnetic-based cell separator
Positive and negative selection for magnetically labeled and unlabeled cells, respectively
Useful for pre-enrichment before FACS
Software
FlowJo V10 (available for use at workstation, please contact us for booking or details)
Summit 4.3 (free version available)
Summit 6.3.1 (free version available)
BD FACS Diva
Beckman Coulter Kaluza (dongle for loan)
CytExpert (free version available)
