Services and Charges
Cell Sorting
Sorter operation
Sorters are prepared
Sample tubes
Suspension samples may be placed in 1.5ml Eppendorf tubes, 12 X 75mm tubes, 15ml or 50ml tubes.
Sample concentration
Recommended cell concentration is 1X106 to 5X106 per ml of buffer/medium.
Beckman Coulter Moflo Astrios can run at 10 000 events per second.
The nozzle size for Astrios is 100um.
Sample filtering
Samples must be filtered with 60um nylon mesh (provided in the facility) to remove clumps that might plug the instrument during sorting
Unfiltered samples will not be allowed to sort
For samples that tend to aggregate over time, you may add some DNase
Add 0.02mg/ml DNase type IIS to all cell preparation steps, including wash steps, to eliminate free DNA from broken cells that leads to aggregation.
Collection
Sorted cells can be collected into
Tubes: 1.5ml Eppendorf tubes, 12 X 75mm tubes, 15ml or 50ml tubes.
Plates: 96-well and 384-well plates
Culture media or buffer or serum may be used in the collection tubes/plates
Tubes/ Plates
Volume of collection media/buffer/serum
1.5ml Eppendorf tube
0.5ml
15ml tube
3ml
50ml tube
5ml
96-well plate
200ul
384-well plate
50ul
It is recommended to spin the plates post sorting for 30-60s at 300G to help the cells to adhere and increase the number of colonies that will grow.
Charges
The table below show the charges for the various equipment. Flow Cytometry Unit also carry a range of related consumables and reagents.
Feel free to contact us to know more!
*All published prices exclude prevailing Goods and Service Tax (GST) and are subjected to changes without prior notice.
| Internal user | External user | |||
| Cell Analysis | ||||
| User Operated | Staff operated | User operated | Staff operated | |
| BD LSRFortessa | $39/hour | $90/hour | $52/hour | $120/hour |
| CytoFLEX LX | $39/hour | $90/hour | $52/hour | $120/hour |
| NovoCyte Advanteon | $39/hour | $90/hour | $52/hour | $120/hour |
| NanoFCM | $50/hour | $120/hour | $65/hour | $150/hour |
| Cell Sorting | ||||
| Moflo Astrios | $70/hour | $96/hour | ||
| Super MACS | $45/ (excluding antibody beads and column) | $60/ (excluding antibody beads and column) | ||
| Data analysis | User operated | Staff operated | ||
| $39/ hour | $90/hour | |||
| Sample preparation | $90/ hour | $120/ hour | ||
Training
All users will be trained by our facility staff and chargeable at $60/pax/equipment.
We also offer chargeable hands-on training programme which provides comprehensive coverage on the field. Please contact Wang Xiaoning (medwx@nus.edu.sg) to confirm the training slot.
In collaboration with industry partners, we also provide advanced training for operators of core facilities located in Southeast Asia.
Programme Outline:
| Activity | Duration |
| Introduction to Flow Cytometry | 60 mins |
| Practical Session: Running samples with analyser (CytoFlex LX or BD LSR Fortessa) | 60 mins |
Samples and some consumables are provided by the facility. The participants may try their own cell samples but please notify us in advance.
Venue:
12 Science Drive 2
Blk MD1, Tahir Foundation Building
Flow Cytometry Laboratory Unit, #B1-03
Singapore 117549
(Take passenger lifts 6, 7, 8 to come down to B1)
Policies
Service Charge and Scheduling Policy
The following rules apply to time scheduled on Flow Cytometry Facility instruments:
- “No-shows” are charged for all of the scheduled time.
- Appointments cancelled less than 24 hours in advance will be charged for the full time.
- Appointments for the cell sorter cannot be scheduled less than 24 hours. 2 to 3 days in advance booking for cell sorting is recommended.
Invoice request
The Facility will generate quarterly invoices that are sent by mail to designated people. Invoices covering other ranges of time can also be generated upon request. If you require certified true copy of invoices, please send your request to medwx@nus.edu.sg .
Data Storage
Data generated by BD LSRFortessa or Cytoflex LX are stored in a database local to the instrument PC. Once a user has completed data acquisition, files can be exported from the database in two formats, either as flow cytometry standard files (FCS) or as entire experiments. It is recommended that FCS files are exported to the instrument PC immediately after the completion of each run for off-line analysis.
Acceptable methods to transfer data:
- Through facility thumbdrive (for Cytoflex LX and BD LSR Fortessa)
We encourage users to back up their data as soon as possible.
We are not responsible for any data loss in the case of computer breakdown or special case etc.
