Service and Charges

Electrophysiology core fee structure

The Core will purchase to provide for the general consumables and reagents that will be proportionately charged to the users. However, users are responsible to supply any materials or reagents that are unique to their particular projects.

Types of service

Guided charge for NUS or NUHS researcher

Guided Charge for non-NUS or NUSH researcher*

Description

Culture and transfection of HEK293 cells for patch clamp

S$120 per experiment

S$180 per experiment

Inclusive of coating of cover slips with PDL, splitting of cells and transfection with lipofectamine 2000 sufficient for two days of recordings

Acute preparation of rodent brain slice

S$80 per experiment

S$120 per experiment

Acute Isolation of mouse cardiomyocytes

S$80 per experiment

S$120 per experiment

Acute isolation of mouse phrenic nerve-diaphragm preparation

S$50 per experiment

S$75 per experiment

Guided charge for NUS researcher/data

Guided Charge for non-NUS researcher/data*

Whole cell patch clamp recording of transfected HEK293 cells

S$30

S$45

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Whole cell patch clamp recording of cultured cardiomyocytes and neurons

S$50

S$75

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Whole cell patch clamp recording of cultured cardiomyocytes and neurons

S$50

S$75

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Whole cell patch clamp recording of brain slice

S$50

S$75

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Electrophysiological analysis of endplate potentials in acutely isolated phrenic nerve-diaphragm preparation

S$50

S$75

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Whole endolysosomal patch clamp electrophysiology of cultured cells

S$100

S$150

Inclusive of whole-cell patch clamp recording and preparation of internal and external solution

Data analysis and figures preparation by the electrophysiology core (experimental design, data acquisition, analysis and interpretation).

Request for authorship contribution

Request for authorship contribution

* 30% increase and 20% additional ind for non-NUS researchers

Prices quoted are subject to change.