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Yong Loo Lin
School of Medicine
Equipment / Capabilities
Electrophysiology
About Us
Equipment / Capabilities
Service and Charges
Events / Workshop / Training
Publication
Contact Us
Electrophysiology
About Us
Equipment / Capabilities
Service and Charges
Events / Workshop / Training
Publication
Contact Us
Social Media

Equipment / Capabilities

Equipment

  1. Nikon Ti inverted fluorescent microscope
  2. Nikon Upright Patch Clamp microscope Model: FN1 with Hammamatsu Orca Flash 4
  3. Manipulator MP-285/ MPC-325-2 (Sutter Instrument Company)
  4. MultiClamp 700B (Axon instruments)
  5. Digidata 1550 Low-Noise Data Acquisition System (Axon instruments)
  6. Vapor pressure osmometer 5520 (VAPRO)
  7. Flaming/brown micropipette puller model P-97 (Sutter Instrument Company)
  8. Micro forge MF-900 (NARISHIGE)
  9. Single Stage Glass Microelectrode Puller MODEL PP-830 (NARISHIGE)
  10. SH27B In-line solution heaters and CC-28 Thermistor (Warner Instruments)
  11. A365 Stimulus Isolator (World Precision Instruments)
  12. Olympus IX81 microscope with Olympus DP71 camera
  13. NEPA21 Electroporator

Key capabilities

  1. Functional characterization and Pharmacological screening of ion channel blockers/activators 
    1. Voltage gated calcium channels (CaV1.2, CaV1.3, CaV2.1)
    2. Voltage gated sodium channels (NaV1.5)
    3. Voltage gated potassium channels (KV1.1, KV1.2, KV1.3, KV7.1, KV11.1)
    4. Two-pore domain potassium channels (TASK-2)
    5. Calcium-activated potassium channels (SKCa, BKCa)
    6. Lysosomal ion channels (TPC1, TRPML1, BKCa)
    7. Acid sensitive ion channels (ASIC2a)
    8. TRP Channels (TRPV1, TRPA1)
  2. Whole cell recording of primary or hIPSC-derived cardiomyocytes or neurons. 
  3. Whole cell recording of neurons within acutely isolated brain slice.
  4. Whole endolysosomal patch clamp electrophysiology of cultured cells
  5. Electrophysiological analysis of endplate potentials in acutely isolated phrenic nerve-diaphragm preparation