This module covers analytical methods and technologies that enable detection and quantification of key molecular descriptors of human health and disease, including proteins, metabolites, and lipids. The first half of the course introduces experimental workflows, instrumentation, and data quality control procedures across different application areas. The second half consists of a series of application-oriented lectures by experts who routinely deploy proteomic and metabolomic profiling methods in their own research areas. We will summarize current practices and future outlook in the context of emerging applications in precision medicine.