Core Facilities
We provide quality, central facility, equipped with cutting edge tools at the forefront of technology
Immunology Translational Research Programme Core Facilities Bioinformatics Core
In the age of single cell RNA sequencing, spatial transcriptomics, multi-omics and deep immunophenotyping flow cytometry, computational immunology data analytics is a necessity. In addition to providing standardized bioinformatics analysis, the core (managed by Dr Chen Jinmiao and Dr Cui Jianzhou) also develops customized bioinformatics analyses for specific projects and provides training to students and staffs. This core also supports the activities of our other facilities including Flow Cytometry, Single Cell Genomics and the NUS-Cambridge Immune Phenotyping Centre.
Our Mission
Data analysis services & Training
Faculty Advisor
Dr Chen Jinmiao, Associate Professor
- Department of Microbiology and Immunology, NUS
- Center for Computational Biology and Program in Cancer and Stem Cell Biology, Duke-NUS Medical School
- Bioinformatics Institute (BII), A*STAR, Singapore
Dr Cui Jianzhou, Senior Research Fellow
- Immunology Translational Research Program
- Consultation
- Analysis
- Follow Up Meeting
Next-generation sequencing data processing:
- DNA sequencing (DNASeq): whole-exome sequencing (WES or WXS), whole-genome sequencing (WGS)
- RNA sequencing (RNASeq): mRNAseq, single-cell RNA sequencing (scRNAseq), microRNAseq, lncRNAseq
- Epigenomics: Methylation sequencing (Methylseq), chromatin immunoprecipitation sequencing (ChIPseq)
- Proteomics: Mass cytometry by the time of flight (CyTOF) and mass spectrometry (MS)
- Metabolomics
- Microbiome
Analyses regularly performed:
- Sample QC and batch correction
- Single nucleotide variants (SNVs), INDELs, copy number variants (CNVs)
- Gene set enrichment and pathways and gene expression
- DEGs and cell types annotation
- Identifying differentiating elements
- Machine learning models for multi-omics integration
- Retrieving and annotating public omics data sets
- Bacteria abundance and diversity
- TCGA, ICGC, Genotype-Tissue Expression (GTEx) project, UK Biobank, Trans-Omics for Precision Medicine (TOPMed), GEO
Developing reproducible workflows, analyzing next-generation sequencing data, and
supporting manuscript development/publication.
Single-cell analysis workshop and Hands on
A, 12th Models of Physiology and Disease Symposium
B, Immunology Research In Progress
- Jianzhou Cui, PhD, Senior Research Fellow
Bioinformatics Core
Immunology Translational Research Programme, Yong Loo Lin School of Medicine
Immunology Programme, Life science Institute
NUS-Cambridge Immunophenotyping centre
National University of Singapore
Center for Life Sciences (CeLS) #03-06C, 28 Medical Dr., Singapore 117456
Email: jianzhou.cui@u.nus.edu