Equipment

Features

• Olympus IX 83 inverted microscope, with motorized XYZ capability.
• 300 hour mercury lamp for fluorescence visualization through eyepiece.
• Filter cube on microscope  :
  • U-FUNA: Ex BP360-370, DM410, Em LP420
  • U-FBNA: Ex BP470-495, DM505, Em BP510-550
  • U-FGWA: Ex BP 530-550, DM570, Em BP575-625

Objectives

• UPLFLN 10x, NA: 0.3
• UPLFLN 20x, NA: 0.5
• UPLSAPO 40x, NA:0.95
• UPLSAPO 60x, NA: 1.35 ( Oil Immersion Lens)

Laser

• Laser Diode : 405nm (50mW); 473nm (15mW); 559nm (15mW); 640nm (20mW)
• Suitable for most dyes/FPs in the visible range including DAPI, Alexa 488, Alexa 568, Alexa 647.
• Uses AOTF for high speed intensity modulation and shutter control

Confocal Scanner

• Silver coated galvanometer scanner capable of image size from 64×64 to 4096×4096
• Optical Scanner Zoom : 1x to 50x.
• Adjustable single pinhole system.

Detector

• 3 CH filter detector unit using Multi-Alkali PMT
• 1 Transmitted detector for BF/DIC
• Imaging of more than 3 FL channels possible by using Virtual Channel Scan.

Software

• Olympus FV10-ASW 4.2a

Applications

• Multi-Dimensional Imaging (X, Y, Z , T)
• Multi-position 2D and 3D Imaging
• Mosaic 2D and 3D Imaging
• Advanced F-techniques: FRAP, FRET
• Photo-stimulation and Photobleaching experiments.