{"id":805,"date":"2020-02-18T06:42:32","date_gmt":"2020-02-18T06:42:32","guid":{"rendered":"https:\/\/medicine.nus.edu.sg\/bch\/?page_id=805"},"modified":"2020-03-04T05:10:01","modified_gmt":"2020-03-04T05:10:01","slug":"examples","status":"publish","type":"page","link":"https:\/\/medicine.nus.edu.sg\/bch\/examples\/","title":{"rendered":"Examples"},"content":{"rendered":"

Examples of approach \/ data generated for various proteomic projects<\/h2>\n

\"MassSpec<\/h2>\n

<\/h2>\n

Post-Translational Modification<\/b><\/h2>\n

Identification of ubiquitination sites<\/b>: Iyengar P, Jaynes P, Rodon L, Lama D, Law KP,\u00a0<\/span>Lim YP<\/b>, Verma C, Seoane J, and Eichhorn P. USP15 regulates SMURF2 kinetics through C-lobe mediated deubiquitination. 2015\u00a0<\/span>Sci. Rep.<\/i>\u00a05:14733<\/span><\/p>\n

Identification of phosphorylation sites<\/b>: Assidiq BF, Tan KY, Toy W, Chong PK and\u00a0<\/span>Lim YP<\/b>. EGFR S1166 phosphorylation induced by a combination of EGF and gefitinib has a potentially negative impact on lung cancer cell growth. 2012\u00a0<\/span>J. Proteome Res<\/i>. 11(8):4110-9<\/span><\/p>\n

Targeted Proteomics<\/b><\/h2>\n

Phosphoproteomics<\/b>: Chen Y, Choong LY, Lin QS, Philps, R, Wong CH, Ang BK, Loh MCS, Hew CL, Shah, N, Druker BJ, Chong PK and\u00a0<\/span>Lim YP<\/b>. Differential expression of novel tyrosine kinase substrates during breast cancer development. 2007\u00a0<\/span>Mol. Cell. Proteomics<\/i>\u00a06(12):2072-87<\/span><\/p>\n

Phosphoproteomics<\/b>: Chen YH*, Low TY*, Choong LY, Rajarshi Sankar Ray, Tan YL, Toy Weiyi, Lin QS, Ang BK, Wong CH, Lim Simin, Li B, Hew CL, Sze NSK, Druker BJ and\u00a0<\/span>Lim YP<\/b>. Phosphoproteomics identified Endofin, DCBLD2 and KIAA0582 as novel targets of EGF signaling and Gefitinib in human cancer cells. 2007\u00a0<\/span>Proteomics<\/i>\u00a07, 2384\u20132397<\/span><\/p>\n

Membrane Proteomics<\/b>: Goh WQJ, Ow GS, Kuznetsov V, Chong PK,\u00a0<\/span>Lim YP<\/b>. DLAT Subunit of the Pyruvate Dehydrogenase Complex is Upregulated in Gastric Cancer \u2013 implications in cancer therapy. 2015\u00a0<\/span>Am J Transl. Res<\/i>. 7(6):1140-1151<\/span><\/p>\n

Membrane Proteomics<\/b>: Yang YX, Toy WY, Choong LY, Hou PL, Ashktorab H, Smoot DT, Yeoh KG and\u00a0<\/span>Lim YP<\/b>. Discovery of SLC3A2 cell membrane protein as a potential gastric cancer biomarker \u2013 implications in molecular imaging. 2012\u00a0<\/span>J. Proteome Res<\/i>. Dec 7;11(12):5736-47<\/span><\/p>\n

Secretomics<\/b>: See LP, Lu S, Chong PK and\u00a0<\/span>Lim YP<\/b>. Increased secretion of CXCL3 as breast cancer cells acquire metastasis potential. 2014\u00a0<\/span>Current Cancer Drug Target<\/i>\u00a0Mar;14(3):294-309<\/span><\/p>\n

Plasma Proteomics<\/b><\/h2>\n

Using human plasma<\/b>: Chong PK, Lee HY, Loh MCS, Lin Q, So J, Lim KH, Soo RA, Yong WP, Chan SP, Smoot DT, Hassan A, Yeoh KG and\u00a0<\/span>Lim YP<\/b>. Up-regulation of plasma C9 protein in gastric cancer patients. 2010\u00a0<\/span>Proteomics<\/i>\u00a010(18):3210-21.<\/span><\/p>\n

Using animal model<\/b>: Chong, PK, Lee, HY, Zhou J, Liu SC, Loh, MCS, Wang TT, Chan SP, Smoot DT, Ashktorab H, So, JBY, Lim KH, Yeoh KG and\u00a0<\/span>Lim YP<\/b>. ITIH3 is a potential biomarker for early detection of gastric cancer. 2010\u00a0<\/span>J. Proteome Res<\/i>. 9(7)-3671\u20133679<\/span><\/p>\n

Using animal model<\/b>: Chong PK, Lee HY, Zhou J, Liu SC, Loh MCS, So JBY, Lim KH, Yeoh KG and\u00a0<\/span>Lim YP<\/b>. Reduced plasma APOA1 level is associated with Gastric Tumor Growth in MKN45 mouse xenograft model. 2010\u00a0<\/span>J. Proteomics<\/i>\u00a073(8):1632-1640<\/span><\/p>\n

Shot-Gun Proteomics<\/b><\/h2>\n

Ophthalmology<\/b>: Hou A, Lan W, Law KP, Khoo SC, Tin MC,\u00a0<\/span>Lim YP<\/b>, Tong L. Evaluation of global differential gene and protein expression in primary pterygium: S100A8 and S100A9 as possible drivers of a signaling network. 2014\u00a0<\/span>PLoS One<\/i>\u00a013;9(5):e97402<\/span><\/p>\n

Bio-Processing<\/b>: Lim UM, Yap MGS ,\u00a0<\/span>Lim YP<\/b>, Goh LT, Ng SK. Identification of Autocrine Growth Factors Secreted by CHO cells for Applications in Single Cell Cloning Media. 2013\u00a0<\/span>J Proteome. Res<\/i>. Jul 2013; 12(7): 3496-510<\/span><\/p>\n

Cancer<\/b>: Choong LY, Lim S, Chong PK, Wong CY, Shah N and\u00a0<\/span>Lim YP<\/b>. Proteome-wide profiling of the MCF10AT breast cancer progression model. 2010\u00a0<\/span>PLoS One<\/i>\u00a0Jun 9;5(6):e11030<\/span><\/p>\n

Cancer<\/b>: Lim S, Choong LY, Chong PK, Chen Y and\u00a0<\/span>Lim YP<\/b>. Regulation of Macrophage Inhibitory Factor (MIF) by Epidermal Growth Factor Receptor (EGFR) in the MCF10AT model of breast cancer progression. 2009\u00a0<\/span>J. Proteome Res<\/i>. 8(8): 4062-4076<\/span><\/p>\n

Cancer<\/b>: Ho J, Kong WFJ, Choong LY*, Loh MCS, Toy W, Chong PK, Wong CH, Wong CY, Shah N and\u00a0<\/span>Lim YP<\/b>. Novel Breast Cancer Metastasis-associated proteins. 2009\u00a0<\/span>J. Proteome Res.<\/i>\u00a08(2):583-594<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Examples of approach \/ data generated for various proteomic projects Post-Translational Modification Identification of ubiquitination sites: Iyengar P, Jaynes P,…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-805","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nExamples - Department of Biochemistry \u2013 School of Medicine, National University of Singapore<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medicine.nus.edu.sg\/bch\/mass-spectrometry-facility__trashed\/examples\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Examples - Department of Biochemistry \u2013 School of Medicine, National University of Singapore\" \/>\n<meta property=\"og:description\" content=\"Examples of approach \/ data generated for various proteomic projects Post-Translational Modification Identification of ubiquitination sites: Iyengar P, Jaynes P,...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medicine.nus.edu.sg\/bch\/mass-spectrometry-facility__trashed\/examples\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Biochemistry \u2013 School of Medicine, National University of Singapore\" \/>\n<meta property=\"article:modified_time\" content=\"2020-03-04T05:10:01+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/medicine.nus.edu.sg\/bch\/wp-content\/uploads\/sites\/13\/2020\/02\/example.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/medicine.nus.edu.sg\/bch\/mass-spectrometry-facility__trashed\/examples\/\",\"url\":\"https:\/\/medicine.nus.edu.sg\/bch\/mass-spectrometry-facility__trashed\/examples\/\",\"name\":\"Examples - 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