{"id":330,"date":"2020-01-21T02:37:14","date_gmt":"2020-01-21T02:37:14","guid":{"rendered":"https:\/\/medicine.nus.edu.sg\/bch\/?page_id=330"},"modified":"2020-06-29T18:40:18","modified_gmt":"2020-06-29T18:40:18","slug":"equipment","status":"publish","type":"page","link":"https:\/\/medicine.nus.edu.sg\/bch\/equipment\/","title":{"rendered":"Equipment"},"content":{"rendered":"<p><u><b>Features<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">Olympus IX 83 inverted microscope, with motorized XYZ capability. <\/span><\/li>\n<li><span lang=\"EN-SG\">300 hour mercury lamp for fluorescence visualization through eyepiece. <\/span><\/li>\n<li><span lang=\"EN-SG\">Filter cube on microscope\u00a0 : <\/span>\n<ul type=\"circle\">\n<li><span lang=\"EN-SG\">U-FUNA: Ex BP360-370, DM410, Em LP420<\/span><\/li>\n<li><span lang=\"EN-SG\">U-FBNA: Ex BP470-495, DM505, Em BP510-550<\/span><\/li>\n<li><span lang=\"EN-SG\">U-FGWA: Ex BP 530-550, DM570, Em BP575-625<\/span><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><u><b>Objectives<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">UPLFLN 10x, NA: 0.3<\/span><\/li>\n<li><span lang=\"EN-SG\">UPLFLN 20x, NA: 0.5<\/span><\/li>\n<li><span lang=\"EN-SG\">UPLSAPO 40x, NA:0.95<\/span><\/li>\n<li><span lang=\"EN-SG\">UPLSAPO 60x, NA: 1.35 ( Oil Immersion Lens)<\/span><\/li>\n<\/ul>\n<p><u><b>Laser<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">Laser Diode : 405nm (50mW); 473nm (15mW); 559nm (15mW); 640nm (20mW)<\/span><\/li>\n<li><span lang=\"EN-SG\">Suitable for most dyes\/FPs in the visible range including DAPI, Alexa 488, Alexa 568, Alexa 647. <\/span><\/li>\n<li><span lang=\"EN-SG\">Uses AOTF for high speed intensity modulation and shutter control<\/span><\/li>\n<\/ul>\n<p><u><b>Confocal Scanner<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">Silver coated galvanometer scanner capable of image size from 64&#215;64 to 4096&#215;4096<\/span><\/li>\n<li><span lang=\"EN-SG\">Optical Scanner Zoom : 1x to 50x. <\/span><\/li>\n<li><span lang=\"EN-SG\">Adjustable single pinhole system. <\/span><\/li>\n<\/ul>\n<p><u><b>Detector<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">3 CH filter detector unit using Multi-Alkali PMT<\/span><\/li>\n<li><span lang=\"EN-SG\">1 Transmitted detector for BF\/DIC<\/span><\/li>\n<li><span lang=\"EN-SG\">Imaging of more than 3 FL channels possible by using Virtual Channel Scan. <\/span><\/li>\n<\/ul>\n<p><u><b>Software<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">Olympus FV10-ASW 4.2a<\/span><\/li>\n<\/ul>\n<p><u><b>Applications<\/b><\/u><span><\/span><\/p>\n<ul class=\"ul-list-style\">\n<li><span lang=\"EN-SG\">Multi-Dimensional Imaging (X, Y, Z , T)<\/span><\/li>\n<li><span lang=\"EN-SG\">Multi-position 2D and 3D Imaging<\/span><\/li>\n<li><span lang=\"EN-SG\">Mosaic 2D and 3D Imaging<\/span><\/li>\n<li><span lang=\"EN-SG\">Advanced F-techniques: FRAP, FRET<\/span><\/li>\n<li><span lang=\"EN-SG\">Photo-stimulation and Photobleaching experiments.<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Features Olympus IX 83 inverted microscope, with motorized XYZ capability. 300 hour mercury lamp for fluorescence visualization through eyepiece. Filter&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-330","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Equipment - Department of Biochemistry \u2013 School of Medicine, National University of Singapore<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medicine.nus.edu.sg\/bch\/equipment\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Equipment - Department of Biochemistry \u2013 School of Medicine, National University of Singapore\" \/>\n<meta property=\"og:description\" content=\"Features Olympus IX 83 inverted microscope, with motorized XYZ capability. 300 hour mercury lamp for fluorescence visualization through eyepiece. 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