{"id":5951,"date":"2024-04-26T15:25:14","date_gmt":"2024-04-26T07:25:14","guid":{"rendered":"https:\/\/medicine.nus.edu.sg\/bch\/?post_type=faculty&p=5951"},"modified":"2024-09-30T20:37:54","modified_gmt":"2024-09-30T12:37:54","slug":"jungjoon-kempthorne-lee","status":"publish","type":"faculty","link":"https:\/\/medicine.nus.edu.sg\/bch\/faculty\/jungjoon-kempthorne-lee\/","title":{"rendered":"Jungjoon Kempthorne Lee"},"content":{"rendered":"
\n

Affiliations<\/h3>\n

Assistant Professor, Department of Biochemistry, Yong Loo Lin School of Medicine, NUS<\/span>
\nPrinciple Investigator, NUS Medicine Synthetic Biology Translational Research Programme<\/span>
\nVice Chairman, Korean Association for Genome Editing<\/span>
\nMember, American Society of Gene & Cell Therapy<\/span>
\nLife Member, The Oxford and Cambridge Society of Singapore<\/span>
\nMember, Stanford Club of Singapore<\/span>.<\/p>\n

<\/h3>\n

Education<\/h3>\n\n\n\n\n\n\n
Degree and Institution<\/b><\/td>\nYear(s)<\/b><\/td>\n<\/tr>\n<\/thead>\n
Ph.D. Stanford University<\/span><\/td>\n2005 – 2011<\/td>\n<\/tr>\n
B.A. M.Sci University of Cambridge<\/span><\/td>\n2001 – 2005<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/h3>\n

Professional Experience<\/h3>\n\n\n\n\n\n\n\n
Position and Institute<\/b><\/td>\nYear(s)<\/b><\/td>\n<\/tr>\n<\/thead>\n
Biotherapeutics Lab, Samsung Advanced Institute of Technology<\/span><\/td>\n2011 – 2014<\/td>\n<\/tr>\n
The Institute of Molecular Biology & Genetics, Seoul National University<\/span><\/td>\n2014 – 2016<\/td>\n<\/tr>\n
Platform R&D, Toolgen<\/span><\/td>\n2016 – 2024<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/h3>\n

Research Interest<\/h3>\n

Advancement of platform technologies for genome editing and broadening their applications.<\/p>\n

<\/h3>\n

Current Research Projects<\/h3>\n
    \n
  1. Investigating the directed evolution of CRISPR and related enzymes. <\/span><\/li>\n
  2. Studying methods for predicting off-target effects across the genome. <\/span><\/li>\n
  3. Developing new genome-editing technologies and identifying their novel uses.<\/span><\/li>\n<\/ol>\n<\/div>\n

    <\/h3>\n

    Selected Publications<\/h3>\n
      \n
    1. Young-hoon Kim, Nahye Kim, Ikenna Okafor, Sungchul Choi, Seonwoo Min, Joonsun Lee, Seung-Min Bae, Keunwoo Choi, Janice Choi, Vinayak Harihar, Youngho Kim, Jin-Soo Kim, Benjamin Kleinstiver, Jungjoon K. Lee*<\/strong>, Taekjip Ha*, Hyongbum Henry Kim*, Sniper2L is ahigh-fidelity Cas9 variant with high activity, Nature Chemical Biology, 2023, https:\/\/doi.org\/10.1038\/s41589-023-01279-5.<\/a><\/li>\n
    2. Jeonghun Kwon, Minyoung Kim, Woochang Hwang, Anna Jo, Gue-Ho Hwang, Minhee Jung, Un Gi Kim, Gang Cui, Heonseok Kim, Joon-Ho Eom, Junho K. Hur, Junwon Lee, Youngho Kim, Jin-soo Kim, Sangsu Bae, Jungjoon K. Lee*<\/strong>, Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches, Genome Biology, 2023, 24, Article number: 4.<\/li>\n
    3. Jeonghun Kwon, Minyoung Kim, Seungmin Bae, Anna Jo, Youngho Kim, Jungjoon K. Lee*<\/strong>,TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor, Nature Communications, 2022, 13, Article number: 7975<\/li>\n
    4. Ikenna C Okafor, Digvijay Singh, Yanbo Wang, Minhee Jung, Haobo Wang, John Mallon, Scott Bailey, Jungjoon K. Lee<\/strong>, Taekjip Ha*, Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding, Nucleic Acids Res. 2019 Dec 16; 47(22): 11880\u201311888<\/li>\n
    5. Jungjoon K. Lee*<\/strong>, Euihwan Jeong, Joonsun Lee, Minhee Jung, Eunji Shin, Young-hoon Kim,Kangin Lee, Inyoung Jung, Daesik Kim, Seokjoong Kim & Jin-Soo Kim*, Directed evolution ofCRISPR-Cas9 to increase its specificity, Nature Communications, 2018; 9, Article number: 3048.<\/li>\n<\/ol>\n","protected":false},"featured_media":5954,"parent":0,"menu_order":15,"template":"","faculty_category":[12,58],"class_list":["post-5951","faculty","type-faculty","status-publish","has-post-thumbnail","hentry","faculty_category-academic-faculty","faculty_category-academic-faculty-academic-faculty"],"acf":[],"yoast_head":"\nJungjoon Kempthorne Lee - Department of Biochemistry \u2013 School of Medicine, National University of Singapore<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medicine.nus.edu.sg\/bch\/faculty\/jungjoon-kempthorne-lee\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Jungjoon Kempthorne Lee - 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