Department of Orthopaedic Surgery

Cell Culture

Location:
MD11, Lab B1-05
Clinical Research Centre
National University of Singapore,
10 Medical Drive, Singapore 117597.

Principal Investigator: Assoc. Prof. Wilson Wang

For Enquiries, please contact:
Lab I/C: Ms Jennifer Chong Sue Wee (doscsw@nus.edu.sg)
Tel: 67724706; Fax: 67744082

 

Cell Culture Lab

The Cell Culture facilities provide various resources for the isolation and culture of animal and human cells. Previous studies have successfully isolated and cultured cells from various musculoskeletal tissues, including bone marrow, periosteum, adipose tissue, tendon and cartilage. The cultured cells are used for upstream research work (molecular and genetic studies) or downstream studies such as in-vivo studies using animals and clinical trials.

The Cell Culture Lab is equipped with:

  • Biological Safety Cabinet (Type II)
  • Incubator for mammalian cell culture
  • Microscope for viewing cell cultures
  • Centrifuge (refridgerated)
  • Freezers (-20 deg C and - 80 deg C)
  • Fume Cupboard
  • Cryogenic tank
  • Autoclave

Cell Culture

Cells isolated from Bone Marrow
Cell Cultured in CO2 Incubator

 

Primary Culture of Bone Marrow
Cell-seeded PCL, BCECF-AM & PI Staining

 

Cells Stained with Hematoxylin and Eosin
Multileanage potential of MSCs