Profile of the Month

Norbert Lehming

Staatsexamen (BSc Hons equivalent), PhD, Habilitation (2000)
Associate Professor
Department of Microbiology and Immunology

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Transcriptional Regulation

The regulation of transcription and the repair of damaged DNA are the topics of interest. Transcription is the basis for the realisation of genetic information, and its regulation is of vital interest for all organisms. All organisms have to be able to repair their DNA when it gets damaged and the inability to repair damaged DNA is a hallmark of cancer. Transcriptional regulation DNA repair are achieved by protein interactions between DNA-bound transcription factors and DNA repair enzymes. Split-Ub, an alternative two-hybrid system, is used to analyse these protein interactions inside living cells. Split-Ub is based upon conditional protein degradation: A protein of interest is fused to the C-terminal half of ubiquitin, which is C-terminally extended by a reporter protein like RUra3p, RGpt2p, or RGFP. In the reporter protein, the first amino acid has been replaced by arginine, an instable residue in the N-end rule pathway of protein degradation.